Leicester City Under-21s will be the only academy side left in the last 16 of the Papa John's Trophy as six others bowed out in the second round on Tuesday.
The under-21 sides at Arsenal, Manchester United, Manchester City, West Ham, Wolves and Norwich all lost to League One and League Two opponents.
Last season's EFL Trophy finalists Salford City, who are still waiting to find out when they will get the chance to face Portsmouth at Wembley in the 2020 showpiece, eventually went down 6-5 on penalties at home against the Foxes youngsters.
It followed an entertaining game which finished 3-3 with Salford substitute Emmanuel Dieseruvwe and Leicester forward Admiral Muskwe both netting twice.