Dean Holden's spell as Bristol City boss ended with a sixth successive defeat as Reading's Veljko Paunovic was left to savour a 2-0 win.
Holden did not conduct his normal post-match interview after Lucas Joao and Michael Morrison fired Reading to victory, and a statement from the club then confirmed his departure.
Holden was under pressure before kick-off following Saturday's 6-0 defeat at Watford. Owner Steve Lansdown had made it clear a rapid transformation in form was necessary if changes at the top were to be avoided.
Holden stepped up from assistant to be made caretaker boss when Lee Johnson was sacked last season and was confirmed as head coach during the summer.