Goalkeeper Zack Steffen will miss the United States' exhibition against Peru, leaving Brad Guzan or Ethan Horvath to start Tuesday in East Hartford, Connecticut.
The U.S. Soccer Federation said Saturday that Steffen has a tight right hamstring and left training camp.
He started the last four U.S. games, including Thursday's 4-2 loss to Colombia in Tampa, Florida.
Guzan is a 34-year-old veteran of two World Cup rosters and 58 international appearances. He is with the national team for the first time since the loss at Trinidad and Tobago last October that ended the Americans' streak of seven straight World Cup appearances.