Fiorentina players Patrick Cutrone and German Pezzella are among three new confirmed cases of coronavirus at the club.
Physio Stefano Dainelli has also tested positive for COVID-19, the Serie A side announced on Saturday.
All three "are in good health at their homes in Florence", Fiorentina said via their official Twitter account.
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On Friday, striker Dusan Vlahovic became the Viola's first confirmed case, although the club said he was at home and did not have any symptoms.
"The club is in the process of undertaking all the necessary isolation procedures in accordance with the regulations, starting with identifying all the people who have had contact with the player," Fiorentina said.