Luis Suarez is out for an indefinite period after injuring his right calf in Barcelona's La Liga loss to Athletic Bilbao on Friday, the club have confirmed.
The 32-year-old striker had to be replaced before half-time at San Mames after receiving treatment following a shot that hit the post.
Lionel Messi missed the match with a calf issue of his own, suffered shortly before Barca travelled to the United States for two friendly matches against Napoli last week.
Editors' Picks
It was the first time in 10 years that Barca started their league campaign without the Argentine and both players' absences were felt as Ernesto Valverde's men succumbed to a stunning late winner from Athletic veteran Aritz Aduriz.