The Matildas went down fighting against world champions United States in their friendly ahead of June's World Cup, losing 5-3 in Denver.
Stunning goals from Lisa De Vanna, Caitlin Foord and Sam Kerr weren't enough for Australia to maintain their winning record under new coach Ante Milicic.
Australia took a 2-1 lead over the world's best team shortly after half-time when Foord twirled her way through midfield to fire home a superb individual effort.
But United States showed their pedigree, storming home with three goals in 13 minutes before withstanding a late flurry in the wake of Kerr's consolation.