Gareth Southgate confirmed Eric Dier will miss England's European Championship qualifier against Montenegro on Monday through injury.
Tottenham midfielder Dier suffered a muscle injury after just 13 minutes of the Three Lions' win against Czech Republic on Friday following a challenge by midfielder Tomas Soucek.
The 25-year-old managed to carry on briefly but was eventually forced off after 26 minutes and replaced by Ross Barkley.
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The former Sporting Lisbon youngster will now return to Tottenham for further treatment with the club facing a nervous wait to see if he will recover in time for their trip to leaders Liverpool in the Premier League next weekend.