The Manchester City star has been ruled out of the tournament due to the illness and will now enter quarantine
Joao Cancelo has been replaced by Diogo Dalot in Portugal's Euro 2020 squad after testing positive for Covid-19.
Cancelo was named in Fernando Santos' final 26-man squad for the European Championships after helping Manchester City win a Premier League and Carabao Cup double in 2020-21.
The 27-year-old was expected to be one of Portugal's key players at the summer tournament, but has been struck down with coronavirus ahead of their Group F opener against Hungary on Tuesday.