Aissata Traore scored the only goal as Besiktas pipped ALG Sport 1-0 in Sunday's final play-off at the Manavgat Atatürk Stadium.
The outcome means the Black and Whites, who were previously back-to-back runners-up, are Bayanlar Ligi women champions for the first time in their history.
Having ended the regular season on level terms with Sunday's title rivals, Mutlucan Zavotcu's side needed a final play-off to clinch their first major silverware in the Turkish top-flight.
It was the Malian's second-half header which helped Zavotcu's ladies become the 10th different side to clinch the Turkish league diadem since it's launch in 1993.