Harry Kane will be among five key Tottenham players rested against Apoel Nicosia on Wednesday but Dele Alli will start, according to Mauricio Pochettino.
Kane, Christian Eriksen, Eric Dier, Kieran Trippier and Hugo Lloris were all absent from training on Tuesday, with Pochettino confirming only those involved at Hotspur Way are in contention.
Spurs have already secured first place in Group H meaning the likes of Fernando Llorente, Georges-Kevin Nkoudou and Juan Foyth are expected to be given a chance in the dead rubber at Wembley.
Nineteen-year-old striker Kazaiah Sterling and 20-year-old midfielder Luke Amos, both academy graduates, will also be in the squad.