Colabello tested positive in April for dehydrochlormethyltestosterone, which is an anabolic steroid also known as oral turinabol. The first baseman receieved an 80-game suspension that ended on Saturday.
The 32-year-old played 10 games in April with the Blue Jays, and hit .069 with one RBI and nine strikeouts. Colabello has been on a rehab assignment since July 13, hitting .189 with a home run, four RBIs and 16 strikeouts in 10 games.
"He's got to play in [live] competition," Blue Jays manager John Gibbons said.
Colabello played in 101 games for the Blue Jays in 2015, helping the ballclub win the American League East make the playoffs for the first time since 1993.