Angelique Kerber, the top overall seed at Wimbledon, is out of the tournament following a 4-6, 6-4, 6-4 loss to No. 14 Garbine Muguruza.
The results ensures that Kerber will lose her No. 1 world ranking after the Grand Slam event. Either Simona Halep or Karolina Pliskova will take over as the top-ranked player after the tournament.
Earlier Monday, Svetlana Kuznetsova became the first player to reach the Wimbledon quarterfinals, as the two-time Grand Slam champion beat Agnieszka Radwanska 6-2, 6-4.
Muguruza and Kuznetsova will square off in the next round.