British two-time champion Andy Murray suffered his earliest Wimbledon exit as he lost in four sets against American John Isner in the second round.
The 35-year-old former world number one clinched the third set to keep his hopes alive but fell 6-4 7-6 (7-4) 6-7 (3-7) 6-4 to the big-hitting 20th seed.
Isner, a 2018 quarter-finalist, fired 36 aces on his way to an impressive victory on Centre Court.
He will play 20-year-old Italian 10th seed Jannik Sinner next.
Murray had never before failed to reach the third round at SW19, in 13 previous attempts.