The Wimbledon men's singles final will not be moved even if England reach the World Cup final on the same afternoon.
The tennis final is scheduled for 14:00 BST on Sunday and the big football match kicks off two hours later.
"We have said for a couple of years that the final will be played at 2pm, as it is every year," said All England Tennis Club boss Richard Lewis.
He also told BBC Sport that Wimbledon's big TV screen on 'Henman Hill' was reserved for tennis.
England face Croatia in the World Cup semi-finals on Wednesday in Russia (19:00 kick-off), with the winner playing France or Belgium on Sunday (16:00).