British number three Katie Boulter's Wimbledon run ended with a 6-3 6-4 defeat by world number 18 Naomi Osaka in the second round.
Boulter, 21, won her first match in the SW19 main draw on Tuesday.
Japan's Osaka, 20, won in 78 minutes on court two and will face 2016 finalist Angelique Kerber or Claire Liu in the third round.
Johanna Konta and Kyle Edmund, the only Britons left in the singles, play later on Thursday.
Konta, 27, plays former world number four Dominika Cibulkova on Centre Court at about 15:30 BST, with Edmund's match against American qualifier Bradley Klahn following.