Australian Open champion Caroline Wozniacki became the biggest name to exit the Wimbledon women's singles as she was beaten in the second round.
The 27-year-old, seeded second, was beaten 6-4 1-6 7-5 by Russian world number 35 Ekaterina Makarova.
The Dane's defeat means five of the top eight women's seeds have exited the competition before the third round.
Wozniacki, who won at Eastbourne last week, has never been past the fourth round in her 12 visits to SW19.
She was plagued by a barrage of flying ants during Tuesday's match on Court One, and needed to use insect repellent to get rid of them.