British number two Heather Watson failed to qualify for the Madrid Open as she lost to Mirjana Lucic-Baroni.
Watson, ranked 19 places higher than the Croat at 56 in the world, led by a break in the first set but Lucic-Baroni came back to win 7-6 (7-4) 6-2.
Naomi Broady, the British number three, plays Puerto Rico's Monica Puig in the final round of qualifying.
Laura Robson will take on Victoria Azarenka in the main draw after using her protected ranking of 58 to enter.
The 22-year-old missed 17 months with a wrist injury, and has won just one WTA main draw match since returning last summer.