James Ward lost 6-4 6-3 to Italy's Matteo Donati in the first qualifying round for the French Open.
British number five Ward, 29, was beaten by a player ranked 99 places below him in the world at 270.
Donati, 21, will play Sweden's Elias Ymer in the next round after moving to within two wins of the main draw at Roland Garros.
Brydan Klein, Britain's other player in the men's qualifiers, plays Ukraine's Sergiy Stakhovsky later on Tuesday.
More to follow.
We've launched a new BBC Sport newsletter ahead of the Euros and Olympics, bringing all the best stories, features and video right to your inbox.