Serena Williams missed four match points before succumbing to Czech seventh seed Karolina Pliskova in a dramatic Australian Open quarter-final.
Williams led 5-1 in the final set but lost the last six games as her bid for a record-equalling 24th Grand Slam singles title ended with a 6-4 4-6 7-5 defeat.
Pliskova took victory on her third match point in Melbourne.
The 26-year-old will meet Japan's Naomi Osaka in Thursday's semi-finals.
Williams' inability to seal victory means a highly anticipated rematch of her controversial US Open final defeat by Osaka must wait.
Like Osaka, Pliskova will be playing in the last four here for the first time.