Emma Raducanu will face fellow Grand Slam winner Sofia Kenin in the Briton's first US Open appearance for two years.
Raducanu memorably triumphed at the US Open as a teenage qualifier in 2021, becoming the first British woman for 44 years to win a major singles title.
She lost in the first round in 2022 and missed last year's tournament after having wrist and ankle surgery.
The 21-year-old faces a potentially tricky task against American Kenin, who won the Australian Open in 2020 and reached the French Open final in the same year.
Katie Boulter, the British women's number one and 31st seed, will start against an as-yet unconfirmed qualifier, while Harriet Dart faces Chloe Paquet of France.