Andy Murray had to retire against Australia's Jordan Thompson with a back injury in what is likely to be his final appearance at Queen's.
It was a sad way for the 37-year-old Briton, who is expected to retire later this year, to leave a tournament which he has won five times.
Murray was playing less than 24 hours after winning his first-round match against Australia's Alexei Popyrin.
From the start, the former world number one looked uncomfortable during the points and was heavily limping between them.
Murray said the issue was not a back spasm, but caused a "loss of strength, coordination and control" in his right leg.