Margaret Court has said "tennis is full of lesbians", following a row over her earlier remarks on gay marriage.
Australian Court, a 24-time Grand Slam singles champion and now a Christian pastor, also said that transgender children were the work of "the devil".
The 74-year-old caused controversy when she said she would not fly on Qantas "where possible" in protest at its support of same-sex marriage.
"When I was playing there was only a couple [of lesbians]," Court said.
"But those couple that led took young ones into parties and things. What you get at the top is often what you'll get right through that sport.