British number one Johanna Konta has been beaten in three sets by Misaki Doi in the Italian Open third round.
Konta, 24, took the opening set but went down 4-6 7-5 6-2 in Rome to her 25-year-old Japanese opponent.
Doi, ranked 45 in the world, belied her position 22 places below the Australian Open semi-finalist to take victory.
Meanwhile, top seed Serena Williams also reached the quarter-finals with a 7-6 (9-7) 6-1 win over fellow American Christina McHale.
Williams will play ninth seed Svetlana Kuznetsova - who beat fellow Russian Daria Gavrilova 6-2 2-6 6-3 - in the last eight, while Doi faces Swiss Timea Bacsinszky.