Katie Boulter and Harriet Dart were among the British players who saw their French Open hopes ended in the second qualifying round on Wednesday.
British number three Boulter lost 6-2 2-6 7-6 (10-3) against American 19-year-old Ashlyn Krueger.
Dart, the British number four, won the first set before falling to a 1-6 6-1 6-2 defeat by France's Elsa Jacquemot.
World number 316 Fran Jones was left in tears after she was forced to retire from her match against Ylena In-Albon.
The 22-year-old, who stunned former grand slam semi-finalist Coco Vandeweghe on Monday, had trailed 4-1.