Defending champion Garbine Muguruza and seven-time Grand Slam winner Venus Williams are out of the French Open, meaning a new major singles champion will be crowned at Roland Garros.
Spanish fourth seed Muguruza, 23, lost 6-1 3-6 6-3 to home favourite Kristina Mladenovic.
American Williams, the 2002 champion, was beaten 5-7 6-2 6-1 by Swiss 30th seed Timea Bacsinszky.
Their last-16 defeats mean only three of the top 10 seeds remain in the draw.
Second seed Karolina Pliskova, third seed Simona Halep and fifth seed Elina Svitolina will aim to reach the quarter-finals when they play their fourth-round matches on Monday.