Angelique Kerber became the first women's top seed to go out in the first round of the French Open when she lost 6-2 6-2 to Ekaterina Makarova.
The German, who also lost in the first round last year, has struggled this year and looked short of confidence.
Makarova, twice a Grand Slam semi-finalist and former top-10 player, dominated rallies from the start.
Kerber improved in the second set but the Russian, now ranked 40th, sealed a famous win in 82 minutes.