Second seed Elina Svitolina and Coco Gauff were beaten in the second round of the Eastbourne International.
American Gauff, 17, started strongly against lucky loser Anastasija Sevastova, but was defeated 4-6 7-5 6-2 by the 31-year-old Latvian.
It was a setback for Gauff the week before Wimbledon, where she had a fairytale run to the last 16 in 2019.
There was also disappointment for Canadian third seed Bianca Andreescu, who was beaten 6-3 6-3 by Estonia's Anett Kontaveit.
Another leading player to make an early exit was Swiss sixth seed Belinda Bencic, who was beaten 6-4 6-4 by compatriot Viktorija Golubic.