Emma Raducanu will not play at next week's Eastbourne International, leaving her short on grass-court preparation before Wimbledon.
Britain's US Open champion managed just seven games against Viktorija Golubic at last week's Nottingham Open before retiring with a side injury.
The 19-year-old had until 16:00 BST on Friday to request a Eastbourne wildcard but did not appear on the list.
Raducanu reached the fourth round at Wimbledon as a wildcard last year.
This year's tournament, where Raducanu will be among the top 10 seeds, begins on 27 June.
Raducanu has struggled with injuries and a lack of consistency since her stunning run to the title in New York last September.