Britain's Emma Raducanu was hampered by a blister on her racquet hand as she was knocked out of the Australian Open in the second round by Danka Kovinic.
The US Open champion raced into a 3-0 lead before needing treatment on her hand and losing the opening set.
The 19-year-old showed remarkable resilience to fight back and win the second but eventually fell to a 6-4 4-6 6-3 defeat on Margaret Court Arena.
Montenegro's Kovinic is into the last 32 of a Grand Slam for the first time.
"It was a really nice experience to play Emma here and make the third round of a Grand Slam for the first time after many years," said Kovinic, the world number 98.