Andy Murray will begin his Wimbledon preparations at the Surbiton Trophy in May before playing at Queen's Club.
The Briton, 35, has opted to skip the French Open to prepare for the grass-court summer.
It will be the first time Murray has played the Challenger Tour event since 2004, when he was just 17-years-old.
British number one and last year's finalist Cameron Norrie will also play at Queen's alongside compatriots Dan Evans and Jack Draper.
Three-time Grand Slam champion Murray has won the singles event at Queen's five times and also triumphed in the doubles with Feliciano Lopez in 2019.