Che Adams, Kenny McLean and Nathan Patterson have dropped out of Scotland's squad for the friendly against Turkey.
Steve Clarke's group are playing in Diyarbakir on Wednesday after failing to reach the World Cup in Qatar.
The Scottish FA did not indicate anyone would be brought in as replacements.
The squad had already been impacted by Celtic's decision not to release players for friendlies during the winter break.
Adams scored at the weekend for Southampton in their 3-1 Premier League defeat at Liverpool, playing the whole game.
Midfielder McLean played all of Norwich City's Championship loss to Middlesbrough at Carrow Road on Saturday, while Patterson completed a full 90 minutes for Everton in a 3-0 defeat away to Bournemouth.