The Roma legend has been handed limited match action under Luciano Spalletti this term but reached the landmark milestone in a convincing home victory over Chievo.
Francesco Totti came off the bench to make his 600th appearance in Serie A for Roma in the 3-0 win over Chievo on Sunday at Stadio Olimpico.
The 39-year-old set up Miralem Pjanic for Roma's third strike of the afternoon following earlier goals from Radja Nainggolan and Antonio Rudiger.
Totti has been forced to settle for a backup role with the Giallorossi this season despite expressing his desire for more first-team football.