The Galaxy and United States great came on in the 83nd minute against Orlando City, returning to the playing field for the first time since winning the 2014 MLS Cup.
Landon Donovan returned to the playing field Sunday nearly two years after announcing his retirement.
With the Galaxy leading 4-1 in the 83nd minute, the team's all-time leader in goals and assists entered the match as a substitute, just days after announcing his return to the Galaxy.
Donovan retired from the sport after the 2014 MLS season, a year that saw him lift the MLS Cup with the Galaxy.