Substitute Stevan Jovetic ended Real Madrid's record-setting, 40-match unbeaten run by netting a stoppage-time winner to give Sevilla a 2-1 victory over Real Madrid at the Ramon Sanchez Pizjuan Stadium on Sunday.
Jovetic, on loan from Inter Milan, lashed a long-range shot into the bottom corner deep into injury time after a Sergio Ramos own goal had let Sevilla equalize. Ramos headed into his own net to earn his former club a foothold.
His own goal had cancelled out Cristiano Ronaldo's successful penalty for Real Madrid in the second half.
The stalemate means La Liga leaders Real Madrid are now just two points ahead of rival Barcelona, although Los Blancos have played a game less.