Federico Bernardeschi has pulled out of the Italy squad, having failed to recover from the muscular injury which has kept him sidelined in recent weeks.
Bernardeschi has missed Juventus' last two Serie A matches and their most recent Champions League fixture against Manchester United with an adductor problem.
He is nearing a return to fitness, having been named on the bench against AC Milan on Sunday, and Roberto Mancini chose to include Bernardeschi in his 27-man squad for matches against Portugal and the United States in the hope that he would be fit to play.