The 30-year-old will now undergo surgery after an MRI scan revealed that he ruptured his anterior cruciate ligament during Juventus' 4-0 win over Palermo.
Juventus midfielder Claudio Marchisio has been ruled out of Italy's Euro 2016 campaign after suffering a cruciate ligament injury.
The 30-year-old was forced off on a stretcher during Juventus' 4-0 victory over Palermo on Sunday after falling awkwardly in a challenge with Franco Vazquez.
Juventus has now confirmed the midfielder has undergone an MRI scan and the results have shown that he has ruptured the anterior cruciate ligament in his left knee, and will now require surgery.