Forest Green Rovers shocked Charlton Athletic on Tuesday in the 2019 Carabao Cup, with the EFL League Two side winning 5-3 on penalties after a goalless draw in the first round against the Championship club.
League One new boys Lincoln City continued their good form with a surprise 1-0 victory at Huddersfield Town.
Crewe Alexander eliminated Middlesbrough 4-2 on penalties after a 2-2 draw at the Riverside Stadium.
Brentford crashed out 5-4 on penalties against Cambridge United after the League Two side earned a 1-1 draw on the road.
Tuesday's Results
Tranmere Rovers 0-3 Hull City
Grimsby Town 1-0 Doncaster Rovers
Wigan Athletic 0-1 Stoke City
Port Vale 1-2 Burton Albion
Nottingham Forest 1-0 Fleetwood Town
Bradford City 0-4 Preston North End
Blackpool 2-2 Macclesfield Town (Macclesfield win 4-2 on penalties)
Blackburn Rovers 3-2 Oldham Athletic
Mansfield Town 2-2 Morecambe (Morecambe win 6-5 on penalties)
Accrington Stanley 1-3 Sunderland
Scunthorpe United 0-1 Derby County
Rochdale 5-2 Bolton Wanderers
Huddersfield 0-1 Lincoln
Middlesbrough 2-2 Crewe (Crewe win 4-2 on penalties)
Shrewsbury Town 0-4 Rotherham United
Salford City 0-3 Leeds United
Barnsley 0-3 Carlisle United
Colchester United 3-0 Swindon Town
AFC Wimbledon 2-2 Milton Keynes Dons (MK Dons win 4-2 on penalties)
Oxford United 1-0 Peterborough United
Queens Park Rangers 3-3 Bristol City (QPR win 5-4 on penalties)
Plymouth Argyle 2-0 Leyton Orient
Wycombe Wanderers 1-1 Reading (Reading win 4-2 on penalties)
Charlton 0-0 Forest Green (Forest Green win 5-3 on penalties)
Gillingham 2-2 Newport County (Newport win 4-1 on penalties)
Stevenage 1-2 Southend United
Luton Town 3-1 Ipswich Town
Walsall 2-3 Crawley Town
Bristol Rovers 3-0 Cheltenham Town
Brentford 1-1 Cambridge (Cambridge win 5-4 on penalties)
Coventry City 4-1 Exeter City
Swansea City 3-1 Northampton Town
West Bromwich Albion 1-2 Millwall
Tuesday Recap
Forest Green, who finished fifth in League Two last term, achieved one of the performances of the round by eliminating Charlton at the Valley.