The Mexico star will get his chance on a huge stage for Los Blancos, with Sergio Ramos manning the midfield.
Javier Hernandez will get his chance to start for Real Madrid in a Champions League quarterfinal second leg against city rival Atletico Madrid.
Real has fallen victim to an injury crisis heading into Wednesday's meeting with Atletico, with Luka Modric, Karim Benzema and Gareth Bale all missing due to injury, while Marcelo is absent due to suspension.
The absences have forced Ancelotti to do some swapping around and use Sergio Ramos in midfield, with Hernandez starting alongside Cristiano Ronaldo and Isco in attack.