Lucas Hernandez is set for a spell on the sidelines after damaging the medial collateral ligament in his right knee in Atletico Madrid's victory over Deportivo Alaves on Saturday.
France international Lucas was forced off in the 35th minute of the 3-0 win at Wanda Metropolitano when Jonathan Calleri landed on his leg following an aerial challenge.
Atletico confirmed an MRI scan showed he suffered a grade two sprain of the MCL, though they offered no timeframe for his return.
Diego Simeone started the match with Saul Niguez at left-back as Filipe Luis, Diego Godin and Juanfran missing out through injury.