Chattanooga FC also advances as Rio Grande Valley SC is no longer eligible.
At the behest of the Houston Dynamo US Soccer made a rule clarification in regards to which pro teams are eligible to enter the US Open Cup in 2016. The adjustments means that Rio Grande Valley is no longer eligible. They are independently owned, but the Dynamo manage all player personnel decisions, so as S2 is not eligible RGVSC is not.
This change means that two teams that would begin in the First Round are no granted byes into the second round.