The biggest night in country music turned into a bit of a roast of Eli Manning -- with Peyton tossing several barbs his little bro's way throughout the evening ... and it was all hilarious!!
The Indianapolis Colts legend was co-hosting the annual CMA Awards show with Luke Bryan in Nashville -- and while a bunch of music stars caught playful shade from the duo ... Eli appeared to find himself as the butt of the jokes more often than not.
In fact, it only took about 10 minutes into the show for Peyton to make his first dig at the former New York Giants star, who he co-hosts his weekly "Monday Night Football" "ManningCast" with.