Brooks, who has won the top prize six times, also beat Chris Stapleton and Eric Church on Wednesday at the Bridgestone Arena in Nashville, Tennessee.
Though he ended the awards show on a happy note, the night was marked by emotional and political moments.
Carrie Underwood broke down while singing during the “In Memoriam” section after photos of the 58 people who died at a country music festival in Las Vegas were shown. Little Big Town’s Kimberly Schlapman quoted Maya Angelou when the foursome won vocal group of the year, while bandmate Karen Fairchild told the audience, “Kindness is an attractive quality.