Rebecca Welch will become the first female referee for a Premier League fixture when she officiates Fulham's match against Burnley on 23 December.
Welch, 40, began refereeing in 2010, and in January became the first woman to referee a men's Championship game.
Sam Allison will also take charge of a top-flight match in December, making him the Premier League's first black referee for 15 years.
He will oversee Sheffield United's match against Fulham on 26 December.
Uriah Rennie - the league's last black referee - retired from officiating in English football's top four divisions in 2009.