Asisat Oshoala got the only goal for Barcelona as they defeated Montpellier 1-0 in Sunday's international club friendly.
The Spanish side were drawn against Juventus in the Women's Champions League Round of 32 on Friday and aimed to end their tour on a high after wins over Espanyol and Arsenal.
The French ladies were hoping to conclude their pre-season campaign on a fine note ahead of their French topflight opener against newly-promoted Stade de Reims.
However, Oshoala's strike just six minutes into the contest at the Stade Bernard Gasset was all Fredrik Bengtsson's side required to secure a morale-boosting win over the hosts.