Villarreal forward Samuel Chukwueze, Watford midfielder Tom Dele-Bashiru and Club Brugge's Kreptin Diatta did not make the Golden Boy 20-man shortlist.
The African trio made the initial 40-man list but they will no longer vie for the accolade that will be decided on December 16 in Turin.
The annual award, presented by Italian sports newspaper Tuttosport, honours the most outstanding young player in Europe under the age of 21.
Since its inception in 2003, no African player has won the award, a jinx the continent will be hoping to end in the next edition.