Cristian Romero and Lisandro Martinez have not been able to join their Argentina team-mates in Miami ahead of a clash with Honduras.
The Premier League centre-backs were unable to register their visas with the US Embassy in London, after it closed in the wake of Queen Elizabeth II's death, according to TyC Sports.
The pair, who turn out for Tottenham and Manchester United respectively, do still have an opportunity to link up with the side before their World Cup squad is announced in November, with visas being processed in Argentina instead.