Barcelona have confirmed Ivan Rakitic has been diagnosed with a hamstring injury.
Rakitic was substituted midway through the second half of Croatia's 3-2 Nations League win against Spain, in which Tin Jedvaj scored a late winner.
Coach Zlatko Dalic subsequently confirmed Rakitic would not travel with Croatia to London for Sunday's decisive clash with England.
And Barcelona announced on Saturday scans have found Rakitic has a hamstring injury in his right leg, with no timescale given for his recovery.
Rakitic was already unavailable for Barca's next game, away to LaLiga title rivals Atletico Madrid on November 24, due to suspension.