James Magnussen, who finished .01 behind Nathan Adrian in the 2012 Olympic 100m freestyle, did not qualify for Rio in the event at the Australian Olympic Trials on Monday.
Magnussen, the 2011 and 2013 World champion, placed fourth at the Australian trials in Adelaide, needing to finish first or second to make the Rio Olympics in the individual 100m free.
Cameron McEvoy won the trials in 47.04 seconds, an Australian record and the fastest-ever time outside of the high-tech swimsuit era. Kyle Chalmers, 17, was second in 48.03, followed by James Roberts (48.