Newcastle defender Paul Dummett has made himself unavailable to play for Wales.
The 25 year old, who is yet to play in a competitive international, has won two caps, both in friendly matches against The Netherlands.
"He doesn't want to play international football which is a disappointment from our point of view," said Wales manager Chris Coleman.
"But he's made that decision, and we have to abide by that."
Wales are third in qualifying group D, four points behind leaders Serbia.