Championship leaders Blackburn saw their perfect record demolished by Reading as they fell to a 3-0 defeat at the Select Car Leasing Stadium.
The odds appeared stacked against the Royals, who were thrashed 4-0 by Rotherham on Saturday, as they lined up against Rovers who had won their first three league games under new boss Jon Dahl Tomasson.
But Paul Ince's side dominated from start to finish and goals from Tom McIntyre, Junior Hoilett and Lucas Joao earned them three points.
Reading handed Championship leaders Blackburn their first defeat under Jon Dahl Tomasson
There was late drama at the bet365 Stadium as substitute D'Margio Wright-Phillips scored a 91st-minute equaliser for Stoke against Middlesbrough.